Journal: Biochemical pharmacology

Article Title: Metabolism of bupropion by baboon hepatic and placental microsomes

doi: 10.1016/j.bcp.2011.04.014

Figure Lengend Snippet: A representative saturation curve of OH-BUP formation by baboon hepatic microsomes. The rate of bupropion hydroxylation to OH-BUP was dependent on bupropion concentration and exhibited typical saturation kinetics.

Article Snippet: The V max and apparent K m values were determined using nonlinear regression analysis of the saturation equation (GraphPad Prism 5, Vision 5.01, Graph Pad Software, Inc.).

Techniques: Concentration Assay

Journal: Biochemical pharmacology

Article Title: Metabolism of bupropion by baboon hepatic and placental microsomes

doi: 10.1016/j.bcp.2011.04.014

Figure Lengend Snippet: The representative saturation curve of threohydrobupropion formation by baboon placental microsomes (A) and the effect of chemical inhibitors selective to carbonyl reductases on the formation of threo- and erythrohydrobupropion by baboon placental microsomes (B). The inhibitors of carbonyl reductase are: 4-methylpyrazole (4-MP, 500µM), barbital (BAR, 500µM), flufenamic acid (FLU, 5µM), dicumarol (DIC, 500µM), menadione (MEN, 100µM), and 18β-glycyrrhetinic acid (18β-GA, 0.1µM). The rates of threo-(TB) and erythrohydrobupropion (EB) are expressed as percent of control (absence of inhibitor) and represent the mean ± S.D. of triplicate experiments. ** Statistical significance of p < 0.01 as determined by one-way ANOVA with Tukey’s comparison.

Article Snippet: The V max and apparent K m values were determined using nonlinear regression analysis of the saturation equation (GraphPad Prism 5, Vision 5.01, Graph Pad Software, Inc.).

Techniques: Control, Comparison